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Taenia disease lowered endemic degrees of proinflammatory cytokines while growing levels of IL-4 along with IL-10, and also the inflamation related migrate to the colon have also been substantially decreased. RT-PCR assays coming from digestive tract indicated that Capital t. crassiceps-infected these animals viewable greater term regarding Arginase-1 but lowered expression regarding iNOS in comparison to DSS-treated uninfected rodents. The actual rates regarding To regulation cells are not increased. The actual adoptive transfer of additionally stimulated macrophages (AAM Phi utes) coming from attacked these animals into mice with DSS-induced colitis reduced the degree of digestive tract swelling. Administration involving indomethacin abrogated your anticolitic effect of Taenia. Therefore, Big t. crassiceps an infection boundaries the actual pathology associated with ulcerative colitis by quelling inflamation related answers mechanistically associated with AAM Phi s and also prostaglandins.Ectopic phrase associated with tropomyosin-related kinase A new (TrkA), your high-affinity receptor regarding nerve expansion factor (NGF), continues to be widely used within mobile or portable culture systems to discover the function in cell survival or perhaps demise situations. In comparison, little is well known in regards to the implications of its phrase in vivo. To address this question, adeno-associated virus (AAV) vectors were utilized expressing TrkA inside the substantia nigra (SN) along with striatum associated with adult rats. Eight several weeks after shift, tyrosine hydroxylase (TH) and also dopamine transporter (DAT) mRNAs ended up a bit diminished inside the ipsilateral SN. This particular lessen had not been longer significant any time NGF has been sent in the striatum. There wasn’t any change involving DAT joining sites or even D1 or perhaps D2 receptor mRNAs as well as binding internet sites inside the striatum, indicating that ectopic TrkA exerts a restricted effect on the pool of TH as well as DAT records, without having affected total dopamine signaling. When transmitted in the striatum, TrkA transgene did not have any relation to how big is the cholinergic interneurons, however it applied standard neurotrophic results, since demonstrated by an enlargement in the projector screen nerves and also nitric oxide synthase (nNOS)-expressing interneurons. This particular trophic motion ended up being made worse by the delivery regarding NGF. Zero poisonous aftereffect of your transgene had been observed. These kinds of data reveal that ectopic phrase associated with TrkA may lead to the particular advertising of neurotrophic effects as well as may influence neuronal plasticity even without exogenous NGF throughout neuronal populations that effortlessly neglect to reply to this specific issue. (c) Next year Wiley Newspapers, Inc.Veruki ML, Oltedal M, Hartveit Elizabeth. Electrical combining and also indirect membrane layer components regarding AII amacrine tissues. L Neurophysiol 103: 1456-1466, 2010. Very first posted The month of january Twenty, The year of 2010; doi: 12.1152/jn.01105.’09. AII amacrine cells inside the mammalian retina are related by means of electric synapses to be able to ON-cone the disease tissues also to additional AII amacrine tissue. To be aware of synaptic incorporation over these interneurons, we need details about the actual junctional conductance (grams(t)), your tissue layer level of resistance (r(mirielle)), the particular tissue layer capacitance (C-m), and also the cytoplasmic resistivity (R-i). Due to extensive power direction, it is difficult to obtain estimates associated with third(mirielle), along with the Q-VD-Oph纯度 relative contribution in the junctional as well as nonjunctional conductances for the total insight opposition of your AII amacrine mobile or portable.

This research researches the consequences involving methionine starvation (Maryland) about sugar subscriber base in rainbow salmon (Oncorhynchus mykiss) hepatocytes. As a consequence, major Captisol生产商 hepatocytes ended up incubated within the presence (+M) or even the shortage (-M) involving methionine regarding Forty-eight l along with glucose usage has been assessed utilizing a book non-radioactive, fluorescent-linked enzymatic analysis. Proof indicated that sugar customer base elevated under methionine starvation, mostly due to improved large quantity involving membrane sure sodium-glucose transporter Only two (SGLT2), which was most likely caused through the cellular reduction in [ATP] due to improved mitochondrial uncoupling, while supported by raised log levels of uncoupling necessary protein 2a (UCP2a). These studies could be the first to point out that the components accountable for the actual fast glucose uptake connected with Mister are usually caused from the increased abundance involving SGLT2 glucose transporter along with mitochondrial uncoupling. (Chemical) 2013 Elsevier Corporation. Just about all legal rights set-aside.C-Terminal carboxylic acid solution made up of inhibitors in the NS3 protease tend to be documented. The sunday paper compilation of straight line tripeptide inhibitors which might be very effective along with selective from the NS3 protease are usually referred to. A substantial info for the effectiveness of the straight line inhibitors hails from the roll-out of any C8 substituent for the B-ring in the quinoline moiety found on the P2 of these inhibitors. The roll-out of the Gemstones methyl class outcomes installing the small boost in the cell-based efficiency of such inhibitors but more importantly within a much better pharmacokinetic profile in test subjects at the same time. Search for C8-substitutions led to your id with the bromo offshoot since the best class with this position, resulting in a substantial surge in your cell-based effectiveness on this type of inhibitors. Structure action studies around the C8-bromo derivatives finally resulted in the invention of clinical choice 30 (Bisexual 201335), a very powerful as well as discerning inhibitor of genotypel NS3 protease using a promising PK profile in subjects.The particular conformational isomerism in the propylene oxide (PO)centre department of transportation center dept of transportation center department of transportation 2-fluoroethanol (Further education) intricate has been looked at making use of molecular order Fourier-transform micro wave spectroscopy complemented rich in stage stomach initio computations. Spinning transitions of three diverse binary conformers are already seen experimentally. On such basis as the particular deal from the new and computed spinning always the same, they could be referred to as these nearly all secure houses, anti – G-g+, anti G+g-, along with syn G+g-. All of the observed structures exhibit an immediate O-H centre dot centre us dot centre dot A hydrogen connect, an intramolecular O-H middle dot centre dept of transportation heart dept of transportation F ree p hydrogen connection and 2 supplementary intermolecular C-H center us dot centre department of transportation middle us dot Y connections. The two anti- conformers, using FE as well as the PO methyl group for the reverse facets with the oxirane band, present greater abundances than the syn conformer. In most about three observed conformers, FIE remains approximately rolling around in its advantageous stream-lined gauche conformation. Your monofluorination from the molecular lock-and-key style program P . o . heart dept of transportation center dept of transportation center us dot ethanol increases not simply the quantity of probable binary conformers, but the splendour power one of them.

The actual device in the postmortem increase in the particular intra-abdominal hemorrhage continued to be unidentified. However, the quantity of bleeding identified during autopsies may not be the same as in which during loss of life. Therefore, the criteria for the proper diagnosis of the main cause of dying within autopsies needs to be very carefully reconsidered. Furthermore, accompanying CT image resolution at the time of loss of life and also possible postmortem changes needs to be carefully considered within postmortem CT image, in order that there’ll stop wrong job from the causes of death. (Chemical) The year 2013 Elsevier Ltd and college involving Forensic and also Authorized Treatments. All rights earmarked.Catalpol, a great iridoid glycoside from different organic resources, has many organic functions. However, the ovarian failure-resistant impact provides scarcely been recently examined. The actual study utilized senile 14-month-old Sprague-Dawley female rats to analyze the actual inside vivo ovarian failure-resistant task regarding catalpol. Day-to-day common ranked doasage amounts associated with catalpol (1, Three, as well as 5 mg/kg/d) with regard to A month considerably greater the degree involving solution 19 beta-estradiol (E-2) along with progesterone (P4) yet diminished follicle-stimulating endocrine and also luteinizing alteration in hormones. Electron microscopic evaluation and stream cytometry indicated that catalpol substantially retarded apoptosis with the ovarian granulocytes with the rodents. These findings suggest that catalpol works on the reproductive organs through healthy ovarian tissue along with bettering both the quality and quantity of roots, as a result bringing about rebalanced E-2 along with P4 quantities within previous test subjects so that catalpol features a primary throughout vivo antiaging influence on your rat ovarian technique.2 new rhodamine types (D (1) and L (Two) ) ended up synthesized, recognized along with their ion reputation house continues to be researched. Each of the particular ionophores exhibit colorimetric along with fluorogenic reply for Hg2+ and also Cr3+ ions amid large numbers of alkali, alkaline world along with cross over steel ions analyzed within acetonitrile. Details scientific studies on resolution of binding continual, joining mode, reversibility associated with presenting, decrease detection restrict have already been performed. Diagnosis involving steel ions inside aqueous press been specifically demonstrated simply by preparation of easy, hassle-free as well as non reusable check cardstock sensors using 2 strategies viz. filtering paper along with tissue layer filtration system loaded with these kinds of ionophores. These two techniques responded greatly for you to both steel ions (Hg2+ and Cr3+) in aqueous remedy, evident through bared-eye. For better feeling with reduced concentration of material ions, reprecipitation then purification enrichment involving ligands in membrane layer filtration system ended up being used.Iwasaki H, Naka Any, Iida KT, Nakagawa Y simply, Matsuzaka To, Ishii Okay, Kobayashi K, Takahashi A, Yatoh S, Yahagi N, Sone polymorphism genetic , Suzuki L, Yamada N, Shimano They would. TFE3 regulates muscle metabolic gene appearance, increases glycogen shops, and boosts the hormone insulin awareness inside mice. Feel M Physiol Endocrinol Metab 302: E896-E902, The coming year. 1st printed Jan 31, Next year; doi: 15.1152/ajpendo.00204.Next year.-The function regarding transcription aspect E3 (TFE3), a new bHLH transcription factor, within immunology and also cancer has become properly characterized.

目的：探讨乳腺癌改良根治术(MRM-BC)中保留肋间臂神经(ICBN)在乳腺癌患者中的效果。方法：选取某院2018年7月-2021年7月诊治的100例女性乳腺癌患者，按随机数字表法分为对照组和观察组各50例，所有患者均行MRM-BC,对照组术中切除ICBN,观察组术中保留ICBN,观察至术后3个月。对比两组上臂内侧感觉障碍发生情况、并发症、生活质量。结果：两组并发症发生率相比，无统计学差异(P>0.05occult hepatitis B infection);术后1、3个月，观察组上臂内侧感觉障碍发生率较对照组低，有统计学差异(P<0.05);术后，观察组生活质量综合评定问卷(GQOLI-74)GSmoothened Agonist试剂QOLI-74内各维度评分较对照组高，有统计学差异(P<0.05)。结MK-2206配制论：MRM-BC中保留ICBN不会增加并发症发生风险，并能够有效减少上臂内侧感觉障碍的发生，有利于感觉功能的恢复，进而提高患者生活质量，安全可靠，临床可大力推行。

目的：研究成纤维细胞生长因子受体2(fibroblastgrowthfactorreceptor2，FGFR2)在肾透明细胞癌（clearcellrenalcellcarcinoma，ccRCC；or kidney renalclearcellcarcinoma，KIRC）中的表达情况，对FGFR2的表达与ccRCC临床病理特Coroners and medical examiners征及预后关系进行分析，并研究FGFR2的表达与其他分子之间作用关系，探讨其在ccRCC发生发展中的作用。方法：从肿瘤基因图谱数据库（TheCancerGenomeAtlas，TCGA）及基因表达综合数据库(GeneExpressionOmnibusdatabase，GEO)中下载ccRCC患者的基因表达及临床信息资料，进行转化及整理。并收集北京大学第一医院泌尿外科104例临床ccRCC样本及癌旁正常组织样本，进行免疫组织化学染色（immunohistochemistry，IHC），并对染色结果进行评分，从而比较ccRCC和癌旁正常组织中FGFR2的表达情况；采用实时荧光定量聚合酶链反应 （quantLGX818采购ifyreal-timepolymerasechainreaction，qRT-PCR）检测正常的肾上皮细胞系（293）和ccRCC细胞系(786O、769P、OSRC-2、Caki-1、ACHN、A498)中FGFR2的mRNA表达水平；对数据库中的ccRCC患者进行进一步分析，研究FGFR2表达与ccRCC患者临床病理特征(包括TNM分期和病理分级)以及生存预后的关系，分析ccRCC患者中FGFR2表达与B细胞、T细胞、自然杀伤（naturalkiller，NK）细胞及中性粒细胞浸润的关系，并使用生物交互数据集通用存储库（BiologicalGeneralRepositoryforInteractionDatasets，BioGRID）构建蛋白质相AZD2281生产商互作用(protein-proteininteraction，PPI)网络，利用网络研究与FGFR2蛋白相互作用的分子。结果：在TCGA数据库中，相较于正常组织样本，FGFR2在ccRCC组织样本中表达下调，在GEO数据库中的表达也呈现出这一差异，并且FGFR2在ccRCC临床样本及cc RCC细胞系中表达下调，此外，FGFR2高表达与较早期的、更低级别的ccRCC相关，并与ccRCC患者较好的预后相关，并且FGFR2表达与B细胞、T细胞、NK细胞及中性粒细胞浸润无明显相关关系。PPI网络显示FGFR2蛋白与某些分子间存在相互作用。结论：本研究揭示了FGFR2 与ccRCC发生发展的潜在作用关系，提示FGFR2可能作为ccRCC患者的预后标志物和潜在治疗靶点。

目的 建立术中预测模型并评估前哨淋巴结阳性及全乳切除的乳腺癌患者非前哨淋巴更多结（NSLN）转移的风险。方法 回顾性收集在国内三甲医院行全乳切除术的601例乳腺癌患者资料，其中建模组221例，内部验证组189例，外部验证组191例。通过Logistic回归分析挖掘影响建模组患者NSLN转移的风险因素，建立多因素模型并进行内外验证。同时比较本研究模型与现有的3个NSLN转移术后预测模型（MSKCC模型、TENON模型及MDA模型）在本研究人群中的应用效果。结果 建模组、内部验证组、外部验证组的NSLN转移率分别是32.6%、32.3%和50.3%。多因素分析显示，建模组中年龄（OR=0.968,95%CI:0.941～0.996）、孕激素受体（PR）状态（OR=0.484,95%CI:0.247～0.951）、肿瘤大小（OR=1.491,95%CI:1.151～1.932）、SLN阳性selleck NMR数量（OR=1.868,95%CI:1.278～2.730）、SLN阴性数量（OR=0.763,95%CI:0.631～0.922）是NSLN转移的影响因素。根据上述指标建立的NSLN转移预测模型在建模组的校准度（Hosmer-Lemeshowχ2=8.309,P=0.404）及区分度[受试者工作特征（ROC）曲线下面积（AUC）=0.752,95%CI:0.685～0.819]均较好。该模型在内部验证组和外部验证组的AUC分别为0.751(95%CI:0.676～0.825)和0.681(95%CI:0.606～0.757)。本模型和MSKCC模型、TENON评分系统的预测价值相当，略优于MDA模型。结论 针对全Biomass sugar syrups乳切除术且SLN阳性的早期乳腺癌患者建立的NSLN转移术中预测模型的内外验证结果均较好，准确度与现有术后预测模型相当。

目的 探讨针灸推拿联合腰椎牵引对腰椎间盘突出症患者疼痛程度与炎性因子水平的影响。方法 选取2019年6月至2021年6月于沭阳县中医院接受治疗的54例腰椎间盘突出症患者，依据随机数字表法将其分为对照组（单纯采用腰椎牵引方式进行治疗）和观察组（在腰椎牵引治疗的基础上联合应用针灸推拿治疗），各27例。两组患者均治疗1个月。对比两组患者CP-690550使用方法治疗后的临床获悉更多疗效，治疗前后视觉模拟疼痛量表（VAS）评分、改良Oswestry功能障碍指数（ODITuberculosis biomarkers）评分、直腿抬高角度及血清炎性因子水平。结果 治疗后观察组患者的治疗总效率高于对照组；与治疗前比，治疗后两组患者的VAS、ODI评分及血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、超敏-C反应蛋白（hs-CRP）水平均降低，且观察组低于对照组，直腿抬高角度均升高，且观察组高于对照组（均P<0.05）。结论 在腰椎牵引治疗的基础上联合针灸推拿治疗腰椎间盘突出症，能够缓解患者疼痛，改善患者下肢功能障碍，降低炎性因子水平，提高治疗效果，促进患者康复。

临床实践表明核磁共振成像对评估胶质瘤非常有价值,它们会提供精确的脑肿瘤成像,便于医生获得精确的脑肿瘤轮廓。通常,影像科专家手动标注分割肿瘤区域,但是专家手动标注会带来标注耗时Captisol NMR,标注差异性等问题。在保留肿瘤图像高分割精度同时,为肿瘤靶区的勾画带来快捷且重复性较高的图像分割方案,成为图像引导放疗技术领域的迫切需求。近年来随着深度学习技术的不断发展,基于深度学习的自动图像分割方法也取得了飞速的发展,为肿瘤靶区自动且高精度分割带来了契机。论文以公开Bra TS数据集的MRI多模态图像为分割对象,利用卷积神经网络来实现并优化脑肿瘤的自动精准分割。通过建立卷积神经网络分割模型,从MRI多模态图像中自动分割出目标区域。本文研究了一种基于UNet++的二维Tibetan medicine图像分割算法。首先将Res Net中的残差模块改进融入U-Net和UNet++中,利用改进的残差模块保留最小特征图包含丰富语义信息的特点,提高网络编码器的特征提取能AY-22989研究购买力。用改进的U-Net对Bra TS 2018的二维切片数据进行训练,因为改进的U-Net和改进的UNet++具有部分相同网络结构,所以引入迁移学习思想,将训练好的改进的U-Net网络权值迁移到UNet++网络中对应相同的位置,实现权值的初始化操作,加快网络收敛速度,同时使分割精度更优。在Bra TS 2018数据集中对二维切片数据进行训练和验证,仿真实验结果证明该方法实现有效的脑肿瘤的分割。对Bra TS 2018的3D切块数据进行训练验证,通过仿真实验证明了改进方法的泛化性,对于3D UNet++网络仍具有良好的效果。在保持目标保真度的同时,设计更加轻量化的3D UNet++网络。首先对MRI图像进行预处理执行标准化、交叉分块和数据增强,从而消除各个模态间的对比度差异,提高网络的泛化能力。然后设计轻量级残差结构替代UNet++中双层卷积结构,使之相较于直接使用双层卷积结构网络,计算量和参数量有了巨幅减少。为了对类残差模块进一步轻量化,本文构建了轻量化类残差结构。最后采用CBAM注意力机制,对长连接后的特征图进行信息筛选,使网络自动关注有效信息,进一步提高网络分割精度。通过对Bra TS 2019的3D切块数据进行测试,本研究所设计的轻量级卷积神经网络相较于直接使用改进的3D UNet++网络,参数量是后者的1/13.4,计算量是后者的1/3.7,参数量减少了31425331,计算量减少了1817.96G,使得实际医疗部署对设备要求更低,推理速度更快。同时,通过实验证明该方法有效地提升了分割精度。

目的对新辅助治疗模式下行根治性低位前切除联合保护性造口的局部进展期直肠癌患者进行调查, 探讨新辅助治疗前至造口还纳后12个月患者的生活质量。方法本研究采用描述性病例系列研究方法。回顾性收集2017年12月至2020年1月期间, 于北京协和医院接受完整新辅助长程放化疗并行根治性低位前切除(LAR)联合保护性造口的中低位局部进展期直肠癌患者。纳入标准:(1)新辅助治疗前行直肠MRI评估为mT3～4b或mN1～2而无远处转移(M0)的患者, 同时肿瘤下缘距肛缘12 cm;(2)新辅助治疗前活检病理确诊直肠腺癌;(3)行完整周期的新辅助治疗;(4)接受根治性保肛手术治疗, 术式均为LAR联合保护性造口, 已经完成造口还纳, 同时随访12个月以上。排除美国麻醉医师协会分级Ⅳ～Ⅴ级、合并多原发结直肠癌者、过去5年内有其他恶性肿瘤病史、需要急诊手术的患者、怀孕或哺乳期女性、有严重精神疾病史者、行核磁共振检查、放化疗或手术治疗禁忌者后, 共纳入83例患者, 男性51例(61.4%), 中位年龄59岁, 体质指数(24.4±3.1)kg/m2medical curricula。采用欧洲癌症研究与治疗机构生活质量问卷的结直肠癌模块(EORTC QLQ-CR29)、国际勃起功能指数(IIEF)、Wexner便秘评分和低位前切除综合征(LARS)量表, 分别调查了解患者新辅助治疗前、术前、造口还纳后3个月、造口还纳后12个月的生活质量变化情况, 包括直肠肛门功能、性功能等方面。量表的各项分值采用M(P25, P75)表示。结果 (1)EORTC QLQ-CR29评分显示, 新辅助治疗前、术前、造口还纳后3个月、造口还纳后12个月, 患者焦虑[分别为64.4(52.0, 82.5)分、75.3(66.0, 89.5)分、82.6(78.0, 90.0)分、83.6(78.0, 91.0)分]和担心身体形象[分别为76.8(66.0, 92.0)分、81.1(76.5, 91.5)分、85.5(82.5, 94.0)分、86.1(82.0, 92.0)分]均有所改善(均P0.01);盆腔痛[分别为5.4(2.0, 8.0)分、5.0(2.0, 7.8)分、3.9(1.0, 5.0)分、3.0(1.0, 5.0)分]、尿失禁[分别为15.7(7.0, 22.0)分、11.1(0, 17.5)分、10.0(0, 17.0)分、9.9(0, 16.0)分]、阳痿[分别为14.3(4.2, 19.0)分、12.2(0, 16.8)分、5.6(0, 10.0)分、5.2(0.2, 8.0)分]、尿频[分别为26.4(13.0, 38.5)分、13.9(0, 20.0)分、13.4(2.5, 21.5)分、13.2(2.0, 20.0)分]和黏液血便[分别为4.7(3.0, 6.0)分、2.6(0, 5.0)分、2.2(0, 5.0)分、1.9(0, 4.0)分]等症状均有改善(均P0.01)。在改善男性性功能、腹痛、口干、担心体质量改变、皮肤疼痛和性交困难方面, 评分存在波动, 但造口回纳后较新辅助治疗前症状均有改善(均P0.05)。在女性性功能、排尿困难、味觉障碍和排粪失禁方面, 造口回纳后较新辅助治疗前差异未见统计学意义(均P0.05)。(2)IIEF量表显示:新辅助治疗模式前后, 各项评分均接近, selleckchem PLX4032差异无统计学意义(均P0.05)。(3)直肠肛门功能量表显示:新辅助治疗前、术前、造口还纳后3个月、造口还纳后12个月, 患者的排气失禁[分别为3.1(0, 4.0)分、2.3(0, 4.0)分、1.8(0, 4.0)分、1.2(0, 3.0)分]和急迫排粪[分别为7.2(0, 11.0)分、5.2(0, 11.0)分、2.9(0, 9.0)分、1.7(0, 0)分]症状均有改善(均P0.01);在改善不完全排空感方面, 患者症状存在波动, 但造口回纳后较新辅助治疗前症状有改善(均P0.05);在需要协助排粪[分别为0(0, 0)分、0.7(0, 1.0)分、0.6(0, 1.0)分、0.7(0, 1.0)分]和排粪失败[分别为0.2(0, 0)分、1(0, 2.0)分、0.8(0, 1.5)分、0.8(0, 1.0)分]方面, 患者症状呈现恶化趋势(均P0.01)。对新辅助治疗效果不同的患者进行分层分析, 比较术前与新辅助治疗前的生活质量改变情况。新辅助治疗较不敏感与较敏感的患者在功能、症状方面改变大致相同, 区别在于, 较不敏感的患者在排尿困难、尿失禁、皮肤疼痛和性交困难方面症状显著改善(均P0.05), 而较敏感患者在便频方面症状改善(P0.05)。结论对于局部进展期直肠癌患者, 新辅助放化疗后行根治性低位前切除联合保护性造口的治疗模式可以从许多方面改善患者生活质量。但需注意患者Z-VAD-FMK价格在需要协助排粪及排粪失败方面, 呈现恶化趋势。

目的 探讨MMPs抑制剂联合环磷酰胺(CTX)干预对结肠癌细胞生物学特性及几丁质酶样蛋白-40(YKL-40)和人类表皮生长因子受体-2(HER-2)表达的影响。方法 人结肠癌细胞株Lo Vo根据实验设置分组为4组:空白对照组(进行溶剂二甲亚砜干预)、CTX干预组(仅进行2μmol/L CTX干预)、GM6001干预组(仅进行2.5μmol/L GM6001干预)和联合干预组(2μmol/L CTX和2.5μmol/L GM6001共同干预)。干预24 h后，采用Realtime PCR检测细胞YKL-40和HER-2 mRNA表达;干预48 h后，蛋白质印迹法检测细胞凋亡蛋白表达;干预72 h后，酶联免疫吸附试验法试剂盒检测细胞上清中YKL-40和HER-2水平;分别于干预24 h、48 h和72 h后，MTT细胞增殖检测试剂盒检测细胞增殖能力。结果 与空白对照组相比，CTX干预组、GM6001干预组和联合干预组细胞YKL-40和HER-2 mRNA和蛋白相对表达量均显著降低，联合干预组也显著低于GM6001ventriculostomy-associated infection干预组和CTX干预组，差异均有统计学意义(P <0.diABZI STING agonist体内实验剂量05)，但GM6001干预组和CTX干预组相比差异无统计学意义(P> 0.05)。与空白对照组相比，CTX干预组、GM6001干预组和联合干预组细胞抗凋亡蛋白Bcl-2相对表达量显著降低，促凋亡蛋白Bax和cleaved-Caspase-3相对表达量均显著增加，差异均有统计学意义(P <0.05);联合干预组Bcl-2表达也显著低于GM6001干预组和CTX干预组，Bax和cleaved-Caspase-3表达也显著高于GM6001干预组和CTX干预组，差异均有统计学意义(P <0.05)，但GM6001干预组和CTX干预组凋亡蛋白表达相比差异无统计学意义(P> 0.05)。干预24～48 h后，空白对照组、CTX干预组和GM6001干预组细胞增殖能力均随时间增加而显著上升，差异均有统计学意义(P <0.05)，而联合干预组不同时间差异无统计学意义(P> 0.05);干预不同时间点，与空白对照组相比，CTX干预组、GM6001干预组和联合干预组细胞增殖能力均显著降低，联合干预组也显著低于GM6001干预组和CTX干预组，差异均有统计学意义(P <0.05)，但GM6001干预组和CTX干预组相比差异无统计学意义(P> 0.05)。结论 MMPs抑制剂联合环磷酰胺干预结直肠癌细胞可通过促进细胞中促凋亡蛋白表达、抑制抑凋亡蛋白蛋白，从而促进结直肠癌细胞凋亡，并可抑制细胞增殖，因此可能进一步抑制结直肠癌发展和恶化，其作用机制可能与抑制细胞中YKL-40和HER-2的表达此网站相关。